Table 1. The sequence of RGAP primers showing polymorphism in resistant (Flanders) and susceptible (Morocco) lines.

Primer	Direction	(5′®3′) Sequence
P1	F (forward)	GGIAAIACIACICTIGCI
P15	R (reverse)	IATGGAICCTTCIGCCATCCA
P1	F	GGIAAIACIACICTIGCI
P4N	R	IAGIGCIAGIGGIAGICC
P19	F	ICCTCCAAAICCAACAAT
P4N	R	IAGIGCIAGIGGIAGICC
P3N	F	GGIGGIGTIGTIGATGAT
P17	R	IAGIGCIAGGGIIAGICC
P5	F	CTTITIGTIGTIGTIGATGAT
P4N	R	IAGIGCIAGIGGIAGICC
P2	F	GTIITGGATGATIRITGG
P10	R	ATGICGCAAGTTGATIAG
P8	F	ATCCTGGTGACIACICGI
P10	R	ATGICGCAAGTTGATIAG
P2	F	GTIITGGATGTITITGG
P9	R	ICGIGTIGTCACCAGGAT

The primer pairs (P1,P15), (P19,P4N), (P1,P4N), (P3N, P17) were designed based on conserved amino acid and nucleotide sequences of LZ-NBS-LRR class of resistance genes including Yr₁₀ (wheat), RPM₁, RPS₂, HTR (arabidopsis), Prf (tomato), RPS₅ (bean), RX (potato) and Mla (barley) genes. The primer pair (P5,P4N) was designed based on conserved sequences of TIR-NBS-LRR class of resistance genes including L₆ and N (cotton) and RPP₅ (arabidopsis) genes. The primer pairs (P2,P10), (P2,P9) and (P5,P10) were designed based on conserved sequences of NBS-LRR class of resistance genes including Cre₃ (wheat), I₂ (tomato) and Bs₂ (pepper) genes; Primers P3N and P4N have been designed by Chen et al. (1998); I: Inosine.